Thus, it was suggested that the MPR Q developed in this study is a promising resin that can efficiently separate large biomacromolecules such as human IgG at higher velocities. Ion exchange Chromatography Resins For Therapeutic Antibodies (mAbs) Purification. 3 Maximum flow velocity that has been verified for long-time use. 2 d 50v is the average particle size of the cumulative volume distribution. After attachment the pKa may be slightly different. Furthermore, a resolution for separation of IgG and BSA by MPR Q was 1.06 at 5.09 cm/min, while it was higher than that for the conventional resin at all linear velocities from 5.09 cm/min to 50.93 cm/min. 1 pKa of protonated octylamine before attachment to the resin. The dynamic binding capacity at 10% breakthrough of MPR Q, determined by frontal analysis, exhibited a capacity of 43.8 mg/cm 3 at 5.09 cm/min and 58% of DBC 10% was maintained even though the linear velocity was increased to 50.93 cm/min. Moreover, the reduced height equivalent to a theoretical plate, h, of human IgG, determined using the linear gradient elution method was 65.8 and was not significantly changed in the range of linear velocities from 20.37 to 50.93 cm/min. Inverse size-exclusion chromatography (iSEC) experiments revealed that the apparent pore diameter of MPR Q was approximately 90 nm, which was sufficiently large for the penetration of human IgG and bovine IgM. Under static conditions, MPR Q exhibited a binding capacity of 49.8 mg-IgG/cm 3-resin at pH 10, whereas the fastest adsorption was observed among the anion-exchanger resins tested. Flexible process design due to large operational window of flow rates and bed heights. Efficient industrial-scale purification based on the well-established Capto platform with traditional ligands. MPR Q resin was prepared by phase separation based on spinodal decomposition followed by dextran grafting and ligand conjugation. Capto Q XP is designed for capture and intermediate purification of large biomolecules, such as immunoglobulins IgG, IgM, IgA, in downstream processes. ne/divinyl benzene resin which has been substituted with quaternary amine groups to yield the strong anion exchanger, Mono Q, or with methyl sulphonate. This study investigated the adsorption capacity and mass transfer properties of a novel macroporous epoxy-polymer-based anion-exchanger, MPR Q, for the efficient separation of therapeutic proteins.
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